Sandbox c2

 1ACJ shows the crystal structure of Torpedo californica acetylcholinesterase (TcAChE) complexed with tacrine. Tacrine is a parasympathomimetic and a centrally acting cholinesterase inhibitor. It was the first centrally-acting cholinesterase inhibitor approved for the treatment of Alzheimer's disease. Tacrine’s ring is stacked between the aromatic rings of tryptophan 84 and phenylalanine 330 (W84 and F330).

Harel M, Schalk I, Ehret-Sabatier L, Bouet F, Goeldner M, Hirth C, Axelsen PH, Silman I, Sussman JL. Quaternary ligand binding to aromatic residues in the active-site gorge of acetylcholinesterase.'

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How do we get the oxygen we breathe?
Oxygen is required by all aerobic animals as a basic mechanism to accept electrons and hydrogen ions produced during metabolism. Even when oxygen is all around us, in the air and in the water, oxygen need to be close to the cells where it's going to be used.

This is the time when blood comes into action. Blood is a specialized body fluid that delivers necessary substances to the body's cells – such as nutrients and oxygen – and transports waste products away from those same cells. Because oxygen is not very soluble in blood, animals have an efficient mechanism for capturing oxygen, transporting, and releasing it by the inner cells: hemoglobin.

Hb, the mighty protein
Hemoglobin (Hb), also spelled haemoglobin, (see on the right a three-dimensional representation of a single molecule) is the protein that carries oxygen from the lungs to the tissues where it's needed. The carbon dioxide produced by the consumption of oxygen. In order to function most efficiently, hemoglobin needs to bind to oxygen tightly in the oxygen-rich atmosphere of the lungs and be able to release oxygen rapidly in the relatively oxygen-poor environment of the tissues. It does this in a most elegant and intricately coordinated way. The story of hemoglobin is the prototype example of the relationship between structure and function of a protein molecule. 

The true colors
As you can see, there are four identical gray, red, blue and orange colored, made-of-balls elements. Let's take a closer look to one of them. This is the 'heme' group, the functional unit of hemoglobin. WAIT: are those the true colors of the heme group? Not really. We are looking at a representation of the real structure, artificially colored following the Corey-Pauling-Koltun scheme (

). Remember: these are artificial representations, using colors, textures, styles and forms chosen with the purpose of helping us to better understand the reality in its rich spacial configuration. This model of the heme group is represented here in a spacefill mode, but we can also draw it as ball and sticks, maintaining always the same spacial structure and color coded information.

Capturing Oxygen and other molecules ...
The "heart" of the hemoglobin is the heme group which is a flat ring molecule containing arbon, itrogen and ydrogen atoms, with a single Fe2+ ion at the center. In a heme molecule, the iron is held within the flat plane by four nitrogen ligands from that ring (rotate the structure with your mouse to see the flat plane from its side). In the proper conditions, an oxygen molecule gets attached to the Fe in the heme group. OBSERVE Are there other changes besides the oxygen being attached to the Fe? We can watch the capturing of an oxygen molecule in the context of a protein single chain or on a close-up view of the isolated Heme group.

And now is when things get interesting. The hem group has the chemical and structural capabilities to capture an oxygen molecule, which happens to be too close to the general shape of a molecule of carbon monoxide, which binds hemoglobin about 240 times faster and better than oxygen, meaning that if both gases are available, hemoglobin will prefer CO over O2. THINK: Can you imagine what will happen if by accident we breathe in a carbon monoxide rich atmosphere?

The whole molecule
Let's go back and take a look to the whole picture. Do you remember the four heme groups in a ribbon-like structure we noticed at the beginning?. This is because the biological active molecule of hemoglobin is a tetramer, this is, a polymer comprising four monomer units: two alpha chains, each with 141 amino acids and two beta chains, each with 146 amino acids. The protein of each of these chains is called globin.

Sickle-cell disease
Sickle hemoglobin differs from normal hemoglobin by a single amino acid: valine (hydrophobic) replaces glutamate (hydrophilic) at position 6 on the surface of the beta chain. This creates an hydrophobic spot. THINK: Why a simple additional hydrophobic spot (actually two spots in the structure WHY?), generated by the change of a single amino acid on a protein with over 500 amino acids becomes so problematic?  On the right, we can see the structure of a deoxygenated hemoglobin, this is, an hemoglobin shortly after releasing the load of oxygen. We can distinguish it's four chains (by it's artificial colors) and the four heme groups with no oxygen attached. This time, the representation is of style spacefill, which is Ok because you know by now that representations are only a different way of drawing a real structure that we can't see.

Both normal and sickle hemoglobin, when in deoxygenated state, have an hydrophobic spot (colored white here) on the beta chains. Two beta chains = two hydrophobic spots on the dehydrogenated hemoglobin. WATCH: Can you find the spots on the two chains?.

The <scene name='User:Jaime_Prilusky/How_do_we_get_the_oxygen_we_breathe/Sickle_hemoglobin/1'>hydrophobic spot present on Sickle hemoglobin sticks to the hydrophobic spot present on dehydrogenized hemoglobin, causing hemoglobin molecules to  <scene name='User:Jaime_Prilusky/How_do_we_get_the_oxygen_we_breathe/Sickle_hemoglobin_chain/1'>aggregate into chains forming long fibers. A <scene name='User:Jaime_Prilusky/How_do_we_get_the_oxygen_we_breathe/Sickle_hemoglobin_chain_close/1'>closer look shows us that Alanine and Leucine from one molecule attract the Valine from another, chaining the two hemoglobin molecules together.

Content advisors
This lesson plan was developed together with Dr. Dvora Cohen, Biology Teacher and Dr Mira Kipnis, Chemistry Teacher, both from the Davidson Institute of Science Education, Weizmann Institute of Science. This page include scenes, structures and ideas from Eric Martz, Frieda S. Reichsman  and Angel Herraez. [[Category:]]